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memglow 640  (Cytoskeleton Inc)


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    Cytoskeleton Inc memglow 640
    Memglow 640, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+probe/bio_rxiv__64898__2026__05__14__722739-277-13-15?v=Cytoskeleton+Inc
    Average 95 stars, based on 28 article reviews
    memglow 640 - by Bioz Stars, 2026-07
    95/100 stars

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    (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

    Journal: medRxiv

    Article Title: Detection and characterization of single SARS-CoV-2 viral particles by flow virometry

    doi: 10.64898/2026.04.28.26351941

    Figure Lengend Snippet: (A) Schematic representation of staining procedure for virus-containing supernatant. Supernatant from infected cells is harvested and centrifugated to remove cellular debris. Staining reagents are added to the sample, fixed with paraformaldehyde (PFA) and diluted in DPBS before being analyzed by flow virometry (FVM). (B) Graphical summary of different labelling techniques for targeting the SARS-CoV-2 spike protein via antibodies, the viral RNA genome via the nucleic acid intercalating dye Syto24 and the viral lipid envelope via the lipid intercalating dye MemGlow-488. (C) Dot plots and MFI of D614G stained with monoclonal antibody Tixagevimab (1 µg/mL; anti-RBD) conjugated to DyLight488 (DL488), with Syto24 (20 µM), with MemGlow-488 (200 nM), respectively.

    Article Snippet: For staining of SARS-CoV-2 particles, cell culture supernatants were incubated with Tixagevimab conjugated to DyLight-488 (1 μg/mL) or Syto24 (20 μM; Thermo Fisher S7559) or MemGlow-488 (200 nM; Cytoskeleton #MG01) for 30 minutes at room temperature (RT).

    Techniques: Staining, Virus, Infection